Microarray analysis/Array-CGH is an efficient and sensitive method of molecular cytogenetics that serves as a detection system for the loss and gain of genomic regions. The resolution limit of conventional cytogenetics is approximately 5 Mb, whereas Microarrays/Array-CGH can detect genomic imbalances with a resolution of < 100 kb. Using specialized Array designs, the resolution can be increased to < 200 bp for specific target regions.
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DNA from patients and reference samples are labeled with different fluorescent dyes and are co-hybridized with immobilized DNA fragments (probes) on a carrier array. These probes are distributed over the human genome. Differences in the copy number lead to a shift of the fluorescent signal of each probe. These signals are detected with a laser scanner. Specialized software is used to assign probes to the respective gene regions and visualize them, a process known as “molecular karyotyping”.
We recommend that you clarify the assumption of costs with your health insurance provider. If needed, we will provide you with a detailed estimation of costs.
Array-CGH analysis is also a useful method for the following investigations:
- Exact characterization of aberrations detected using classical cytogenetics
- Verification of aberrations that appear to be balanced
- Characterization of genomic rearrangements
- Additional analysis of molecular genetic processes (e.g., secondary characterization of MLPA results, validation of NGS results)
- 3–5 ml EDTA blood or 5 µg of genomic DNA
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Processing Time: 2–4 weeks