Raw sequencing data are automatically processed in order to facilitate immediate genetic evaluation. Included in delivery are demultiplexed, and adapter trimmed FastQ files. Further options for bioinformatic analyses are available:
- Alignment of trimmed sequencing data (BAM format)
- Normalization (DESeq2, FPKM)
- Quantitative expression profiles (TSV format)
- Differential gene expression (TSV format)
- Gene ontology annotation
- Group comparisons
At CeGaT, paired-end sequencing (2x100bp) is performed using the state-of-the-art Illumina NovaSeq6000 Sequencing System.
If customers require sequencing parameters other than those presented in our product portfolio, please let us know! We can provide further solutions.