Located on the surface of T-cells, the T-cell receptor (TCR) is relevant for the recognition of antigens presented by the major histocompatibility complex (MHC) molecules on antigen-presenting cells. Through somatic rearrangements T-cells express a broad range of unique receptors. These highly diverse heterodimers are mostly composed of two subunits, the α and β chains and to a low percentage of γ and δ chains.
The TCR α and γ chains are generated by V/J recombination (random rearrangement of the variable (V) and joining (J) genes). β and δ chains are generated by V/J/D recombination, where diversity (D) genes are included in addition.
In response to antigen recognition the individual TCR repertoire is shaped by VDJ recombination. This results in an extremely diverse complementary-determining region 3 (CDR3). This region is an attractive target to assess the overall TCR repertoire diversity, given that it is thought to be unique to each TCR- ß variant.
CeGaT´s TCR sequencing service offers the ability to investigate almost the entirety of the T-cell repertoire. We provide our customers with information about diversity, clonality as well as changes in the repertoire or single TCR clones (TCR clone tracking) for different time points.
Investigation of TCR repertoire can provide:
- insights into functions of T-cells in immune response, e.g. immunosuppression
- monitoring drug therapies, such as immunotherapies in cancer as well as the related change in T cell status
- improvement of personalized medicine by tumor-infiltrating T-cell analysis