CeGaT´s TCR sequencing service offers the ability to investigate almost the entirety of the T-cell repertoire. We provide our customers with information about diversity, clonality as well as changes in the repertoire or single TCR clones (TCR clone tracking) for different time points.
Investigation of TCR repertoire can provide
insights into functions of T-cells in immune response, e.g. immunosuppression and immune response
monitoring drug therapies, such as immunotherapies in cancer as well as the related change in T-cell status
improvement of personalized medicine by tumor-infiltrating T-cell analysis
Located on the surface of T-cells, the T-cell receptor (TCR) is relevant for the recognition of antigens presented by the major histocompatibility complex (MHC) molecules on antigen-presenting cells. Through somatic rearrangements T-cells express a broad range of unique receptors. These highly diverse heterodimers are mostly composed of two subunits, the α and β chains and to a low percentage of γ and δ chains.
The TCR α and γ chains are generated by V/J recombination (random rearrangement of the variable (V) and joining (J) genes). β and δ chains are generated by V/J/D recombination, where diversity (D) genes are additionally included.
In response to antigen recognition the individual TCR repertoire is shaped by VDJ recombination. This results in an extremely diverse complementary-determining region 3 (CDR3). Given that the CDR3 is mainly unique to each TCR-ß variant, this region is mostly used to classify overall TCR repertoire.
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We are happy to discuss sequencing options and find a solution specifically tailored to your clinical study or research project.
You help us by specifying sample information including starting material, number of samples, preferred library prep option, preferred sequencing depth and required bioinformatics.
Sequencing data are delivered within 15 – 20 working days. A fast track option is available upon request. The turnaround time (TAT) highly depends on the provided specimen or the quality of DNA and RNA and the requested bioinformatics.
Your sequencing data will be available for download from one of our servers. request, as well as for large sample batches, data can also be delivered via external hard drive, or uploaded to your own server.
The original samples and, if applicable, the isolated nucleic acids will be stored at CeGaT for a period of 3 months after data delivery.
Data will be stored for 5 months.
Raw sequencing data are automatically processed in order to facilitate immediate genetic evaluation. Included in delivery are demultiplexed, and adapter trimmed FASTQ files.
Basic bioinformatic analysis for each project includes:
Reconstruction of TCR clones
Clonality and diversity estimation
Detailed project report
Further options for bioinformatic analyses are available on request such as:
repertoire changes across different time points
We offer our clients T-cell Receptor Sequencing of the ß chain including CDR3.
Choose your target to be examined on DNA or RNA level.
With TCR DNA the determination of relative abundance of clonotypes (sensitive quantification) is possible
TCR RNA enables the detection of expression levels of the repertoire
With CeGaT´s longstanding expertise in genetic diagnostics our clients benefit from examining a variety of sample types like PBMCs, whole blood, fresh frozen tissue and FFPE tissue.
At CeGaT, paired-end sequencing (2 x 100 bp) is performed using the state-of-the-art Illumina NovaSeq 6000 Sequencing System.
If you require sequencing parameters other than those presented in our product portfolio, please let us know! We can provide further solutions.