Small RNA sequencing focuses on short RNA molecules which play an important role in silencing and post-transcriptional gene expression regulation processes. Sequencing of small RNAs allows the analysis of these molecules in more detail and therefore can be an essential part in the identification of novel biomarkers to obtain more valuable research data.
“We have selected the most suitable library preparation protocol for our customers”
For establishing our small RNA sequencing service, three small RNA library preparation protocols (X, Y, Z) were considered and assessed according to different aspects. Here we present the establishment of our favorite sequencing protocol and discuss the reasons why we think this protocol is the best choice.
A very important aspect for small RNA sequencing is the option to prepare libraries from very limited amounts of sample material. For one of the chosen library preparation kits (X) the lowest input amount is 100 ng of total RNA compared to 1 ng total RNA for the other two kits (Y, Z). An additional advantage of protocols Y and Z is the gel-free size selection during library preparation, whereas kit X requires a size selection via gel purification. Due to the lack of a low input option, library preparation kit X was excluded from further investigations.