Sample Requirements

Please quantify the concentration of your sample using a fluorometric based method (e.g. Qubit). The quality of your sample should be further assessed by Bioanalyzer or detecting the optical density., resulting in an OD 260/280 ~ 2.0. RNA samples need to be DNase treated.

*The cycle threshold (CT) in reverse transcription polymerase chain reaction (RT-PCR). Assessment of viral RNA using a qPCR assay.

Please quantify the concentration of your sample using a fluorometric based method (e.g. Qubit). The quality of your sample should be further assessed by Fragment Analyzer or detecting the optical density. We recommend using DNA samples with high molecular weight DNA that were RNase treated resulting in an OD 260/280 of 1.8 – 2.0. Of course, also fragmented DNA can be sequenced when library is prepared according to our special protocols.

All isolated DNA samples should be delivered in molecular biology grade water, 10 mM Tris-HCl pH 8 or Elution Buffer, free of EDTA.

When we shall use your own custom read primer for the sequencing of your library please send 30 µl of 100 µM concentrated primer.

General sample shipment recommendations:

• DNA samples should be shipped chilled at 4° C or frozen.
• RNA samples should be shipped frozen on dry ice.
• Sequencing libraries should be shipped frozen on dry ice.

Please inquire about submitting lower input amounts or degraded sample material