Examine a distinct set of regions within the genome
A gene panel covers a distinct set of regions within the genome. Prior to high throughput sequencing, the selected set of target genes is enriched and only these selected targets will be sequenced. With this method, genes of interest can be examined in many samples at once with a high coverage and a reduced percentage of underrepresented regions. Furthermore, only smaller and more manageable data sets are needed for analysis.
CeGaT has been using this concept in genetic diagnostics since 2009. Based on our long-standing expertise we can offer our predesigned CeGaT Panels for your research project or assist you in individual Custom Panel design with subsequent panel validation.
Turnaround time
Sequencing data are delivered within 20-30 working days. A fast track option is available upon request.
Delivery times for panels and the time needed for validation depends on the complexity of the panel you want to design with us. You will of course receive information about the planned project timeline as soon as we have discussed the project details with you.
Delivery
Your sequencing data will be available for download from our server.
Upon request, as well as for large sample batches, data can also be delivered via external hard drive, or upload to your own server.
Storage
The original samples and, if applicable, the isolated nucleic acids will be stored at CeGaT for a period of 3 months after data delivery.
Your data will be stored for 5 months after data delivery.
Explore Our Panel Sequencing Product Portfolio
PAS Classic
Sequencing panel
Predesigned CeGaT Panel
Species
Human
Panel produced by
Twist Bioscience
Sequencing technology
Illumina
Deliverables
Project report & FASTQ files
PAS Flex
Sequencing panel
Custom Panel
Species
Flexible
Panel produced by
Twist Bioscience
Sequencing technology
Illumina
Deliverables
Project report & FASTQ files
PAS: Panel Sequencing
All products can be combined with further bioinformatics.
Bioinformatics
For CeGaT Panels sequencing data are automatically processed to facilitate immediate genetic evaluation. You will receive the following:
- Demultiplexed and trimmed raw sequence data (FASTQ format)
- Alignment of sequencing data (BAM format)
- Calling of variants (SNVs) as well as insertions and deletions (indels) (VCF format)
- Annotation of SNVs and indels (based on various public data bases) (TSV format)
- Calling of copy number variations (CNVs) and large homozygous deletions (TSV format)
Comparison of affected genes between multiple samples can be offered on request as well as other statistical evaluations.
When you design your Custom Panel with us, the data format for delivery and individual data analysis will be defined depending on your needs.
Technical Information
When you choose one of our predesigned CeGaT Panels you can rely on our expertise in sequencing. The CeGaT Panel design is based on scientific evaluation of the respective disease, and panels are updated on regular basis. More than 99 % of the targets of all CeGaT Panels are sequenced with at least 30x coverage.
Individual Custom Panels can also be designed according to your personal request. We can assist you in target definition and will validate your individual Custom Panel according to diagnostic grade quality approved standards: CAP and ISO:15189.
We suggest paired-end sequencing of CeGaT Panel-enriched libraries (2x 100 bp) using the state-of-the-art Illumina NovaSeq 6000 Sequencing System. If you require sequencing parameters other than those presented in our product portfolio, please let us know! We can provide further solutions.
Contact Us
Start Your Project Now
We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library prep option, preferred sequencing depth and required bioinformatics, if possible.
Further Reading
