Molecular tumor analysis and liquid biopsy: a feasibility investigation analyzing circulating tumor DNA in patients with central nervous system lymphomas.

Authors

Hickmann AK1,2, Frick M3, Hadaschik D3, Battke F3, Bittl M4, Ganslandt O4, Biskup S3,5,6, Döcker D3,6.
  1. Department of Neurosurgery, Kantonsspital St. Gallen, Rorschacherstrasse 95, 9600, St. Gallen, Switzerland. anne-
    katrin.hickmann@kssg.ch.
  2. Neurosurgical Department, Klinikum Stuttgart, Stuttgart, Germany. anne-katrin.hickmann@kssg.ch.
  3. Center for Genomics and Transcriptomics (CeGaT) GmbH, Tübingen, Germany.
  4. Neurosurgical Department, Klinikum Stuttgart, Stuttgart, Germany.
  5. Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany.
  6. Outpatient Clinic for Human Genetics, Tübingen, Germany.

Abstract

BACKGROUND:
Central nervous system lymphomas (CNSL) is a devastating disease. Currently, a confirmatory biopsy is required prior to treatment.

OBJECTIVE:
Our investigation aims to prove the feasibility of a minimally-invasive diagnostic approach for the molecular characterization of CNSL.

METHODS:
Tissue biopsies from 6 patients with suspected CNSL were analyzed using a 649gene next-generation sequencing (NGS) tumor panel (tumor vs. reference tissue (EDTA-blood)). The individual somatic mutation pattern was used as a basis for the digital PCR analyzing circulating tumor DNA (ctDNA) from plasma and cerebrospinal fluid (CSF) samples, identifying one selected tumor mutation during this first step of the feasibility investigation.

RESULTS:
NGS-analysis of biopsy tissue revealed a specific somatic mutation pattern in all confirmed lymphoma samples (n = 5, NGS-sensitivity 100%) and none in the sample identified as normal brain tissue (NGS-specificity 100%). cfDNA-extraction was dependent on the extraction-kit used and feasible in 3 samples, in all of which somatic mutations were detectable (100%). Analysis of CSF-derived cfDNA was superior to plasma-derived cfDNA and routine microscopic analysis (lymphoma cells: n = 2, 40%). One patient showed a divergent molecular pattern, typical of Burkitt-Lymphoma (HIV+, serologic evidence of EBV-infection). Lumbar puncture was tolerated without complications, whereas biopsy caused 3 hemorrhages.

CONCLUSIONS:
Our investigation provides evidence that analysis of cfDNA in central nervous system tumors is feasible using the described protocol. Molecular characterization of CNSL could be achieved by analysis of CSF-derived cfDNA. Knowledge of a tumor’s specific mutation pattern may allow initiation of targeted therapies, treatment surveillance and could lead to minimally-invasive diagnostics in the future.