The 16S ribosomal RNA (rRNA) gene is approximately 1500 bp long and contains several conserved and hypervariable regions (V1-V9) that vary between different bacteria. These hypervariable sequences can be used to identify and characterize microbial diversity. Therefore, the 16S rRNA gene is a common marker to characterize microbial communities in various specimens.
Using the PacBio single molecule real-time (SMRT) technology, we accurately sequence the full-length 16S rRNA gene, covering all variable regions with an average HiFi read length of about 1.5 kb. The extraordinary accuracy and length of PacBio HiFi reads that is achieved by circular consensus sequencing (CCS) allows microbial taxa detection at high resolution.
Advantages of Full-Length 16S Sequencing:
- Characterized by fast speed and high precision
- Analysis of the complete 16S rRNA gene (V1-V9 regions)
- HiFi read length of about 1.5 kb
- Analysis of multiple samples in a single sequencing process
Applications of Full-Length 16S Sequencing are diverse and include:
- Characterization of different microbial communities
- Microbial biomarker detection
- Disease monitoring
- Drug development