Small RNA sequencing focuses on short RNA molecules which play an important role in silencing and post-transcriptional gene expression regulation processes. Sequencing of small RNAs allows the analysis of these molecules in more detail and therefore can be an essential part in the identification of novel biomarkers to obtain more valuable research data.
CeGaT´s small RNA sequencing service offers reliable and accurate insights into small RNAs from extraction to data analysis. With our long-standing expertise in the diagnostic field, our clients benefit from strict quality control standards and therefore high-quality results.
Sequencing data are delivered within 15 – 20 working days. A fast track option is available upon request. The turnaround time (TAT) highly depends on the quality of the provided RNA and the requested bioinformatics level.
Your sequencing data will be available for download from one of our servers (FTAPI or SFTP). Upon request, as well as for large sample batches, data can also be delivered via external hard drive or uploaded to your own server.
The original samples, and, if applicable, the isolated nucleic acids will be stored at CeGaT for a period of 3 months after data delivery.
Data will be irrevocably deleted after 5 months.
Raw sequencing data are automatically processed in order to facilitate immediate genetic evaluation. Included in delivery are demultiplexed and adapter trimmed FastQ files.
Further options for bioinformatic analyses are available for human samples:
Alignment of trimmed sequencing data (BAM format)
Counting of miRNAs
Normalization with miRge
Differential gene expression (reads can be mapped to known miRNAs and pre-miRNAs)
Annotation with miRBase
Small RNA sequencing is performed using the state-of-the-art Illumina NovaSeq6000 Sequencing System, single end (1x 50 nt) with at least 10 mio reads per sample.
If customers require sequencing parameters other than those presented in our product portfolio, please let us know! We can provide further solutions.
Explore our Small RNA Sequencing product portfolio