Ready to Load Sequencing

Research & Pharma Solutions

Information

CeGaT offers sequencing of Illumina compatible Ready to Load next-generation sequencing (NGS) libraries. We make use of the latest NGS technology. Depending on the required output, samples will be sequenced on the MiSeq or NovaSeq 6000 Illumina platform.

Flexible sequencing options are available, specifically tailored for your project. Please refer to our technical information for further details.

Choosing CeGaT for your project, you will benefit from our broad experience in sequencing as well as our high quality standards, based on our German DAkkS ISO 15189 and American CAP/CLIA accreditation.

Start your project now

We are happy to discuss sequencing options and find a solution specifically tailored to your sequencing project.

In order to sequence your libraries as fast as possible and deliver the best sequencing data, please indicate the library preparation kit, number of pooled samples, barcodes / indices that have been used, and information about custom primers.

Turnaround time

Sequencing data are delivered within 5 – 10 working days.

A fast track option is available upon request.

Storage

The original samples will be stored at CeGaT for one week after data delivery.

Data will be irrevocably deleted after 5 months.

Bioinformatics

Depending on your requirements, you will either receive raw sequencing data (BCL files) or demultiplexed FASTQ files. If needed, adapter trimming can be performed. Further bioinformatic analyses are available upon request.

Delivery

Up to 70 Gigabyte of data will be available for download from our download portal FTAPI, a secure end-to-end encrypted system. Upon request, as well as for large sample batches, data can also be delivered via external hard drive, or uploaded to your own server.

Technical Information

At CeGaT, sequencing is performed using the state-of-the-art Illumina NovaSeq 6000 Sequencing System, as well as the MiSeq Sequencing System.

As starting material, we accept any Illumina-compatible library. Illumina index sequencing primers, as well as custom primers may be used during the sequencing process at CeGaT.

If you have generated libraries with commercial library construction kits for PCR-based targeted sequencing using a unique molecular identifier (UMI), we can offer sequencing modes with customized sequencing parameters. The lengths of read 1 and read 2, as well as index read lengths can be adapted to your needs.

Explore our Ready to Load sequencing product portfolio with various sequencing options for your libraries. Depending on the batch size, required output and read number specifications, you can choose from different flow cells.

NovaSeq 6000 platform (Illumina)

SP flow cell

SR/PE

100 cycles

65 Gb

650 MM Single reads CPF*

SR/PE

300 cycles

200 Gb

650 MM Single reads CPF*

PE

500 cycles

325 Gb

650 MM Single reads CPF*

S1 flow cell

SR/PE

100 cycles

134 Gb

1330 MM Single reads CPF*

SR/PE

200 cycles

266 Gb

1330 MM Single reads CPF*

SR/PE

300 cycles

400 Gb

1330 MM Single reads CPF*

S2 flow cell

SR/PE

100 cycles

333 Gb

3335 MM Single reads CPF*

SR/PE

200 cycles

667 Gb

3335 MM Single reads CPF*

SR/PE

300 cycles

1000 Gb

3335 MM Single reads CPF*

S4 flow cell

SR/PE

200 cycles

1600 Gb

8000 MM Single reads CPF*

SR/PE

300 cycles

2400 Gb

8000 MM Single reads CPF*

SR: Single-Read, PE: Paired-End, CPF: clusters passing filter.
*In case of PE sequencing, they will be twice as much.

MiSeq platform (Illumina)

Nano flow cell

SR/PE

300 cycles

0.3 Gb

1 MM Single reads CPF*

PE

500 cycles

0.5 Gb

1 MM Single reads CPF*

Micro flow cell

SR/PE

300 cycles

1.2 Gb

4 MM Single reads CPF*

V2 flow cell

SR/PE

50 cycles

0.75 Gb

12 MM Single reads CPF*

SR/PE

300 cycles

4.5 Gb

12 MM Single reads CPF*

PE

500 cycles

7.5 Gb

12 MM Single reads CPF*

V3 flow cell

SR/PE

150 cycles

3.3 Gb

22 MM Single reads CPF*

PE

600 cycles

13.2 Gb

22 MM Single reads CPF*

SR: Single-Read, PE: Paired-End, CPF: clusters passing filter.
*In case of PE sequencing, they will be twice as much.

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