Information
The Human Leukocyte Antigen (HLA) system is a group of genes which have an important function in the human immune system. The HLA region is highly polymorphic and contains six classical HLA genes: class I HLA-A, -B and –C, and class II HLA-DRB1, -DQB1 and -DPB1. Furthermore, the HLA region is known to be associated with more than 100 multifactorial, complex diseases mainly of inflammatory and autoimmune pathogenesis. In order to find out more about the functions and complex interactions of the HLA system, next-generation sequencing (NGS) based HLA typing can be very helpful.
NGS can for example support characterisation of individual response to drug therapy including pharmacogenetics, HLA-matching of donor-recipient pairs for transplantation, or deliver important information for immunotherapy to fight cancer. A huge advantage of NGS in HLA research is the possibility of fast and precise identification of HLA alleles.
Turnaround time
Sequencing data are delivered within 15 – 20 working days.
A fast track option is available upon request.
Delivery
Your sequencing data will be available for download from one of our servers.
Upon request, as well as for large sample batches, data can also be delivered via external hard drive, or uploaded to your own server.
Storage
The original samples and, if applicable, the isolated nucleic acids will be stored at CeGaT for a period of 3 months after data delivery.
Data will be stored for 5 months on our system after data delivery.
Explore our HLA Typing product portfolio
HLA class I typing
HLA type
HLA class I (A, B, C)
Species
Human
DNA quality
High molecular weight DNA
Protocol
Whole Exome Sequencing (Twist Bioscience)
Evaluation software
OptiType
Sequencing technology
Illumina NovaSeq 6000
Read length
2 x 100 bp
Output
50x mean coverage
Deliverables
Project report & FASTQ, XLS, PDF, TXT files
Combined HLA class I and HLA class II typing
HLA type
HLA class I (A, B, C), HLA class II (DR, DQ, DP)
Species
Human
DNA quality
High molecular weight DNA
Protocol
TruSight HLA Sequencing Panel (Illumina)
Evaluation software
Assign TruSight HLA
Sequencing technology
Illumina MiSeq
Read length
2 x 150 bp
Output
100x mean coverage
Deliverables
Project report & FASTQ, XLS, PDF, TXT files
All products can be combined with our DNA isolation service. In addition, a fast track option is available.
Bioinformatics
For HLA class I typing based on WES, OptiType algorithm is used for data evaluation.
For HLA class I and HLA class II typing based on Panel Sequencing, HLA analysis is performed with Assign TruSight HLA analysis software (Illumina).
You will receive the HLA typing results as Excel (.xlsx) and Text (.txt) file. Raw data will be provided as demultiplexed and adapter trimmed in fastq.gz files.
Technical Information
In order to provide our customers with the best adjusted solution for HLA typing, two different HLA typing options are available:
- HLA class I typing:
Analysis is performed with a special algorithm (OptiType) based on Whole Exome Sequencing data, generated by next generation sequencing using the Illumina NovaSeq 6000 Sequencing System (2x 100bp). The following loci will be analyzed: HLA-A, HLA-B and HLA-C. - Combined HLA class I and HLA class II typing:
Sequencing libraries are generated with the TruSight HLA v2 Panel (Illumina). Libraries will be sequenced on the Illumina MiSeq Sequencing System (2x 150 bp). Data evaluation is done with the Assign TruSight HLA software (Illumina). The following 11 HLA loci are covered by the panel: HLA-A, HLA-B, HLA-C, HLA-DRB1/3/4/5, HLA-DQB1, HLA-DPB1, HLA-DQA1, HLA-DPA1.
Contact Us
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We are happy to discuss sequencing options and to find a solution specifically tailored to your clinical study or research project.
When getting in contact, please specify sample information including starting material, number of samples, preferred library prep option, preferred sequencing depth and required bioinformatics, if possible.
Further Reading
