Fgf9Y162C Mutation Alters Information Processing and Social Memory in Mice.

Authors

Garrett L1,2, Becker L2,3, Rozman J2,3,4, Puk O1,2,5, Stoeger T2,6, Yildirim AÖ2,6, Bohla A2,6,7, Eickelberg O2,6, Hans W2,3, Prehn C2,3, Adamski J3,8, Klopstock T9,10,11, Rácz I12, Zimmer A12, Klingenspor M13, Fuchs H2,3, Gailus-Durner V2,3, Wurst W1,10,11,14, Hrabě de Angelis M2,3,4,8, Graw J1,2, Hölter SM15,16.
  1. Institutes of Developmental Genetics, Helmholtz Zentrum München, Neuherberg, Germany.
  2. German Mouse Clinic, Helmholtz Zentrum München, Neuherberg, Germany.
  3. Experimental Genetics, Helmholtz Zentrum München, Neuherberg, Germany.
  4. German Center for Diabetes Research (DZD), Neuherberg, Germany.
  5. CeGaT GmbH, Paul-Ehrlich-Str. 23, 72076, Tübingen, Germany.
  6. Comprehensive Pneumology Center, Institute of Lung Biology and Disease, Helmholtz Zentrum München, The German
    Center for Lung Research (DZL), German Research Center for Environmental Health, Neuherberg, Germany.
  7. Satorius Stedim Biotech, Aubagne, France.
  8. Experimental Genetics, Faculty of Life and Food Sciences Weihenstephan, Technische Universität München, Freising
    Weihenstephan, Munich, Germany.
  9. Department of Neurology, Friedrich-Baur Institute, Klinikum der Ludwig-Maximilians-Universität München, Munich, Germany.
  10. Deutsches Zentrum für Neurodegenerative Erkrankungen e. V. (DZNE), Munich, Germany.
  11. Munich Cluster for Systems Neurology (SyNergy), Adolf-Butenandt-Institut, Ludwig-Maximilians-Universität München,
    Munich, Germany.
  12. Institute of Molecular Psychiatry, Medical Faculty, Universität Bonn, Bonn, Germany.
  13. Molecular Nutritional Medicine, Faculty of Life and Food Sciences Weihenstephan, Technische Universität München, Munich,
    Germany
  14. Developmental Genetics, Faculty of Life and Food Sciences Weihenstephan, Technische Universität München, Freising
    Weihenstephan, Munich, Germany.
  15. Institutes of Developmental Genetics, Helmholtz Zentrum München, Neuherberg, Germany. hoelter@helmholtz
    muenchen.de.
  16. German Mouse Clinic, Helmholtz Zentrum München, Neuherberg, Germany. hoelter@helmholtz-muenchen.de.

Abstract

In neuropsychiatric diseases, such as major depression and anxiety, pathogenic vulnerability is partially dictated by a genetic predisposition. The search continues to define this genetic susceptibility and establish new genetic elements as potential therapeutic targets. The fibroblast growth factors (FGFs) could be interesting in this regard. This family of signaling molecules plays important roles in development while also functioning within the adult. This includes effects on aspects of brain function such as neurogenesis and synapse formation. Of this family, Fgf9 is expressed in the adult brain, but its functional role is less well defined. In this study, we examined the role of Fgf9 in different brain functions by analyzing the behavior of Fgf9Y162C mutant mice, an Fgf9 allele without the confounding systemic effects of other Fgf9 genetic models. Here, we show that this mutation caused altered locomotor and exploratory reactivity to novel, mildly stressful environments. In addition, mutants showed heightened acoustic startle reactivity as well as impaired social discrimination memory. Notably, there was a substantial decrease in the level of adult olfactory bulb neurogenesis with no difference in hippocampal neurogenesis. Collectively, our findings indicate a role for the Fgf9Y162C mutation in information processing and perception of aversive situations as well as in social memory. Thus, genetic alterations in Fgf9 could increase vulnerability to developing neuropsychiatric disease, and we propose the Fgf9Y162C mutant mice as a valuable tool to study the predictive etiological aspects.