Explore cellular diversity with 10X Genomics Single-Cell Technology

CeGaT expands its product portfolio in the business area Research and Pharma Solutions with 10X GENOMICS’ single-cell sequencing technology. Using this unique technology, the gene expression of thousands of single cells can be analyzed by encapsulating them in tiny water-in-oil droplets. Sequencing the 10X NGS-libraries on one of our Illumina sequencing platforms provides excellent single-cell transcriptome data to discover new biomarkers, rare cell types, and much more.

Human body cells are extremely heterogeneous concerning their transcriptional state, even if they originate from the same organ or tissue. Traditional bulk RNA sequencing methods reach their limits in resolving this heterogeneity.  CeGaT’s Single-cell RNA Sequencing enables the analysis of individual transcriptomes from thousands of cells in a single sample.

Application areas of Single-cell RNA Sequencing:

  • Detection of tumor heterogeneity
  • Cell Differentiation & Lineage Tracing
  • Response to therapeutic intervention
  • Biomarker Discovery

Using the state-of-the-art 10x Genomics® Chromium™ System and the latest NGS technology, CeGaT is able to profile 500 – 10,000 individual cells per sample. With the Chromium™ System, up to 8 samples can be analyzed in parallel. Sequencing is performed using the Illumina NovaSeq 6000 and MiSeq sequencing systems. This allows CeGaT the maximum flexibility in sequencing.

CeGaT’s Single-cell RNA Sequencing Workflow is highly flexible and can be customized to the number of cells in your sample and your desired sequencing depth.

Deliverables for each Single-cell RNA Sequencing project are:

  • Raw sequencing data (FASTQ files)
  • A standard Loupe Browser visualization file
  • Mapped count tables
  • A preliminary clustering and differential gene expression analysis
  • A project report, including QC values and results of the data analysis

We would be happy to discuss all options for your clinical trial or research project.

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