The transcriptome is the entirety of all RNAs present in a specific tissue at a distinct time point. Their presence and abundance correspond to the current metabolic state of the cells and show reactions to external and internal changes. Sequencing the transcriptome allows the detection and quantification of RNA molecules. Application areas and objectives for transcriptome sequencing in the scientific field are diverse and range from the analysis of differential gene expression to the detection of alternative splicing and previously unknown transcripts. Transcriptome sequencing is carried out in basic research as well as in applied clinical research.
We offer the sequencing of the whole transcriptome, as well as sequencing of specific classes of RNA molecules such as coding RNA (mRNA) and small RNAs.
Total RNA Sequencing Service
By using total RNA sequencing, all coding and non-coding RNA molecules are detected. Ribosomal RNAs make up a large proportion of total RNA molecules. Some applications require their removal before sequencing. Thus we offer ribosomal RNA depletion for many common organisms as an additional service.
Small RNA sequencing focuses on short RNA molecules. It allows to discover novel miRNA and other small noncoding RNA molecules and to study their relative abundances. Such molecules are often part of silencing and post-transcriptional gene expression regulation processes. The sequencing of small RNAs can be a vital part in the identification of novel biomarkers.