Customized FISH-Assays

Along with the increasing number of projects utilizing next-generation sequencing (NGS) we expect the identification of a large number of novel amplified, deleted, or translocated genes playing a key role in tumor initiation and progression. The gold standard for in-situ validation of such genes is fluorescence in-situ hybridisation (FISH). Unfortunately, only a limited spectrum of commercially available FISH probes is available.

To close this gap, CeGaT together with Prof. Perner, Professor and Chairman of the Pathology Network of the University Hospital of Lübeck and Leibniz Research Center Borstel, offers the development of FISH assays for in-situ detection of amplifications, deletions, and translocations for nearly all gene loci, tailored to individual needs.

Availability

On request we perform the in situ hybridization and the evaluation of your material.

Immediately available

24 different Break-Apart FISH Probe Kits and

Immediately available selection of break-apart FISH probe kits

Gene Chrom. Location OMIM
ADCY2 5 p15.31 103071
ADCY3 2 p23.3 600291
ALK 2 p23.2 – 23.1 105590
BRAF 7 q34 164757
CREBBP 16 q34 164757
E2F4 16 q22.1 600659
ELK4 1 q32.1 600241
EML4 2 p21 607442
EP300 16 q13.2 602700
ERG 21 q22.2 165080
ETV1 7 p21.2 600541
ETV4 17 q21.31 600711
ETV5 3 q27.2 601600
KRAS 12 p12.1 190070
NDRG1 8 q24.22 605262
POMC 2 p23.3 176830
PTK6 20 q13.33 602004
RAF1 3 p25.2 164760
RET 10 q11.21 164761
RUNX1 21 q22.12 151385
SLC45A3 1 q32.1 605097
SOS1 2 p22.1 182530
TMPRSS2 21 q22.3 602060
ZNF142 2 q35 604083

Locus-specific FISH Probes

Immediately available selection of locus-specific FISH probes

Gene Chrom. Location OMIM
ABCC1 16 p13.11 158343
AR x q11-q12 313700
DCUN1D1 3 q26.33 605905
ETV1 7 p21.2 600541
FAS 10 q23.31 134637
FBXO25 8 p23.3 609098
FBXO3 11 p13.11 609089
FBXO9 6 p12.1 609091
FGFR1 8 p12.1 136350
FOXF1 16 q24.1 601089
GOLPH3 5 p13.3 612207
KRAS 12 p12.1 190070
MET 7 q31.2 164860
MMP16 8 q21.3 602262
MYC 8 q24.12-q24.13 190080
NEK2 1 q32.3 604043
NKX3-1 8 p21.2 602041
PARD3 10 p11.22-p11.21 606745
PDK3 X p22.1 300580
PPM1D 17 q23.2 605100
PTEN 10 q23.31 601728
PTK2 3 q24.3 600758
SOX2 3 q26.33 184429
TITF1 14 q13.3 600635
USP24 1 p32.3 610569
WHSC1L1 8 p11.23 607083
YAP1 11 q22.1 606608
YWHAZ 8 q22.3 601288
ZNF217 20 q13.2 602967

We are happy to develop customized FISH probes. You may obtain a detailed consultation by Prof. Perner. The created customized FISH probe can be applied for at least 20 tests. Reorders of the same FISH probe are possible at any time.

The duration of the development of a FISH probe is four to six weeks after order. Please contact us for an offer.

For questions you can always send an email to Prof. Perner – FISH@cegat.de.